Flag-HA-USP48
(Plasmid #22585)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pDEST_LTR_N_FLAG_HA_IRES_puro
  • Backbone manufacturer
    Harper_Lab
  • Backbone size w/o insert (bp) 6521
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    USP48
  • Alt name
    ubiquitin specific peptidase 48
  • Alt name
    DKFZp762M1713, MGC132556, MGC14879, RAP1GA1, USP31
  • Species
    H. sapiens (human)
  • Mutation
    None
  • GenBank ID
    BC104896
  • Entrez Gene
    USP48 (a.k.a. USP31, RAP1GA1, MGC14879, MGC132556, DKFZp762M1713)
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site GATEWAY_LR (unknown if destroyed)
  • 3′ cloning site GATEWAY_LR (unknown if destroyed)
  • 5′ sequencing primer CAGCCCTCACTCCTTCTCTAGG
  • 3′ sequencing primer CAAGCGGCTTCGGCCAGTAAC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    MGC/Open
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-HA-USP48 was a gift from Wade Harper (Addgene plasmid # 22585)
  • For your References section:

    Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, Bennett EJ, Gygi SP, Harper JW. Cell. 2009 Jul 23. 138(2):389-403. 10.1016/j.cell.2009.04.042 PubMed 19615732