Addgene - pEF1a-mRor2WT Plasmid Data

Plasmid 22613: pEF1a-mRor2WT

Gene/insert name:
Ror2 WT
Alt name:
Ror2
Insert size:
2835
Species:
M. musculus (mouse)
GenBank ID:
AB010384
Entrez Gene:
Ror2 (Ntrkr2, mRor2)
Fusion protein or tag:
Flag
Terminal:
C terminal on insert
Vector backbone:
pEF1a-myc/his A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
9226
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
BGH reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Dr. Y. Minami (Japan)
Sequence: View sequences (1)
Principal Investigator:
Roel Nusse
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Ror2 receptor requires tyrosine kinase activity to mediate Wnt5a signaling. Mikels et al (J Biol Chem. 2009 Aug 31. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22613" in your Materials and Methods section.