pHisrKr2
(Plasmid #22698)

• Depositing Lab: Lynn Hudson
• Publication: Lovas et al Glia. 2001 Apr 15. 34(2):110-20.

Backbone

• Vector backbone: pET-16b
• Backbone size w/o insert (bp): 5711
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha (normal growth conditions)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rat Kruppel-type protein 2
• Alt name: rKr2
• Species: R. norvegicus (rat)
• Insert Size (bp): 492
• Entrez Gene: Zfp111 (a.k.a. rKr2)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 terminal primer
• 3′ sequencing primer: T7 promoter

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The Rat rKr2 DNA was cloned in Hudson laboratory

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pHisrKr2 was constructed to express a part of rKr2 as a histidine-tagged recombinant protein in E. coli for antibody production. This rKr2 DNA fragment encodes amino acids 48-211 of the protein.