MSCV P2Gm +SwaI
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22699||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneMSCV2.2 with WPRE cloned into ClaI site is the base vector
- Backbone size w/o insert (bp) 7500
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Growth instructionsgrow in stbl3 cells at 30oC
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
See FLIP vector protocols (PDF from this page) for more information.
This vector contains the SwaI site for cloning multiple shRNAs into a single vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV P2Gm +SwaI was a gift from Richard Hynes (Addgene plasmid # 22699)