cyclin D1-HA-pBabe-Hygro
(Plasmid #22703)

• Depositing Lab: William Kaelin
• Publication: Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Backbone

• Vector backbone: pBabe-Puro
• Backbone size w/o insert (bp): 5536
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha or XL1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cyclin D1
• Alt name: CCND1
• Species: H. sapiens (human)
• Insert Size (bp): 1159
• Mutation: wt cDNA
• GenBank ID: M64349
• Entrez Gene: CCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)
• Tag / Fusion Protein:
  • HA-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: Sal I (not destroyed)
• 5′ sequencing primer: pBabe-5'
• 3′ sequencing primer: pBabe-3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cyclin D1 cDNA insert from pBabePuro-CyclinD1 kindly provided by Dr. P. Sicinski, DFCI This insert is most probably derived from Addgene plasmid # 9050 (pBABE puro cyclinD1 HA ), deposited by William Hahn, DFCI

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry