|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22730||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS+
- Backbone size w/o insert (bp) 2995
Vector typeBAC recombineering
Selectable markersdiphtheria toxin A
Growth in Bacteria
Growth instructions1. EL350 – for recombineering transformation steps 2. DH5alfa, XL1-blue, or other competent bacterial cell line – for non-recombineering transformation steps
Copy numberHigh Copy
Insert Size (bp)1069
MutationThis is a gap-repair vector designed for retrieving the targeted region of BAC clones to construct gene targeting vectors using a recombineering assembly strategy. The plasmid contains a diphtheria toxin selection gene, DT-A, cloned between the Kpn I and Sal I restriction enzyme sites of pBluescript KS+.
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn I (not destroyed)
- 3′ cloning site Sal1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMCS.DT-A was a gift from Mark Magnuson (Addgene plasmid # 22730 ; http://n2t.net/addgene:22730 ; RRID:Addgene_22730)
For your References section:Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA. Dis Model Mech. 2011 Feb 14. ():. 10.1242/dmm.006569 PubMed 21324933