Addgene - pBp-Control Sponge (CXCR4 sponge) Plasmid Data

Plasmid 22744: pBp-Control Sponge (CXCR4 sponge)

Gene/insert name:
CXCR4 control sponge
Alt name:
stable control sponge
Insert size:
2000
Vector backbone:
pBABE-puro
(Search Vector Database)
Backbone manufacturer:
Weinberg lab (Addgene#:1764)
Vector type:
Mammalian Expression, Retroviral
Backbone size (bp):
5169
Modifications to Backbone:
Non-targeting complementary sites (7x in tandem) to serve as a negative control for microRNA sponge-mediated inhibition of specific microRNA function; suitable for stable expression. Note that EGFP is also cloned into this backbone, upstream of the sponge.
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
ApaI
Site destroyed during cloning:
Unknown
5' sequencing primer:
pBabe-5 List of Sequencing Primers
3' sequencing primer:
pBabe-3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Subclond from CMV-CXCR4 control sponge, as described in Ebert et al. (Nature Methods, 2007)

Sequence of sponge: AAGUUUUCAGAAAGCUAACA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis. Valastyan et al (Cell. 2009 Jun 12. 137(6):1032-46. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22744" in your Materials and Methods section.