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pAc5.1A-Dm-miR-13a
(Plasmid #22761)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22761 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAc5.1A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5348
  • Vector type
    Drosophila expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Dh5 alpha and XL1-blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    micro RNA 13a
  • Alt name
    miR-13a
  • Species
    D. melanogaster (fly); primary transcript
  • Insert Size (bp)
    382
  • GenBank ID
    NR_002027
  • Entrez Gene
    mir-13a (a.k.a. Dmel_CR32931, CR32931, Dmel\CR32931, MiR-13a, miR-13, miR-13a, miR13a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Ac5 forward primer site
  • 3′ sequencing primer BGH reverse priming site
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1A-Dm-miR-13a was a gift from Elisa Izaurralde (Addgene plasmid # 22761)
  • For your References section:

    Genome-wide analysis of mRNAs regulated by Drosha and Argonaute proteins in Drosophila melanogaster. Rehwinkel J, Natalin P, Stark A, Brennecke J, Cohen SM, Izaurralde E. Mol Cell Biol. 2006 Apr . 26(8):2965-75. 10.1128/MCB.26.8.2965-2975.2006 PubMed 16581772