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pcDNA3-K9 histone methylation reporter
(Plasmid #22866)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22866 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    K9 HMR
  • Insert Size (bp)
    1722
  • Tags / Fusion Proteins
    • CFP (N terminal on insert)
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site AflI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-K9 histone methylation reporter was a gift from Alice Ting (Addgene plasmid # 22866 ; http://n2t.net/addgene:22866 ; RRID:Addgene_22866)
  • For your References section:

    Genetically encoded fluorescent reporters of histone methylation in living cells. Lin CW, Jao CY, Ting AY. J Am Chem Soc. 2004 May 19. 126(19):5982-3. 10.1021/ja038854h PubMed 15137760