Addgene - p3xFLAG-CMV10-hAtg101 Plasmid Data

Plasmid 22877: p3xFLAG-CMV10-hAtg101

Gene/insert name:
Atg13-interacting protein
Alt name:
ATG101
Insert size:
657
Species:
H. sapiens (human)
Entrez Gene:
C12orf44 (ATG101, FLJ11773)
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Vector backbone:
p3xFLAG-CMV10
(Search Vector Database)
Backbone manufacturer:
Sigma
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6299
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV 30 sequencing primer List of Sequencing Primers
3' sequencing primer:
CMV 24 sequencing primer
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Noboru Mizushima
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Atg101, a novel mammalian autophagy protein interacting with Atg13. Hosokawa et al (Autophagy. 2009 Oct . 5(7):973-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22877" in your Materials and Methods section.