peGFP-Chk1
(Plasmid #22888)

• Depositing Lab: Aziz Sancar
• Publication: Unsal-Kacmaz et al Mol Cell Biol. 2005 Apr . 25(8):3109-16.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Chk1
• Species: H. sapiens (human)
• Insert Size (bp): 1430
• GenBank ID: CHEK1
• Entrez Gene: CHEK1 (a.k.a. CHK1, OZEMA21)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: EGFP-N Sequencing Primer

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The following residue changes were found during quality control: D130A and L257P. These mutations are of no known functional significance.