Addgene - pcDNA4-Chk1-Flag Plasmid Data

Plasmid 22894: pcDNA4-Chk1-Flag

Gene/insert name:
Chk1
Alt name:
CHEK1
Insert size:
1430
Species:
H. sapiens (human)
GenBank ID:
AK300082.1
Fusion protein or tag:
Flag
Terminal:
C terminal on insert
Vector backbone:
pcDNA4a
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5100
Cloning site 5':
EcoRI
Site destroyed during cloning:
Unknown
Cloning site 3':
PmeI
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Zeocin
Person or lab that originally
cloned the gene/insert:
Stephen Elledge; pBSChk1
Sequence: View sequences (3)
Principal Investigator:
Aziz Sancar
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement. Unsal-Kacmaz et al (Mol Cell Biol. 2007 Apr . 27(8):3131-42. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22894" in your Materials and Methods section.