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pcDNA4-Chk1-Flag
(Plasmid #22894)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA4a
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Chk1
  • Alt name
    CHEK1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1430
  • GenBank ID
    AK300082.1
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site PmeI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Stephen Elledge; pBSChk1
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4-Chk1-Flag was a gift from Aziz Sancar (Addgene plasmid # 22894)
  • For your References section:

    The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement. Unsal-Kacmaz K, Chastain PD, Qu PP, Minoo P, Cordeiro-Stone M, Sancar A, Kaufmann WK. Mol Cell Biol. 2007 Apr . 27(8):3131-42. 10.1128/MCB.02190-06 PubMed 17296725