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pME18S-3HA-mULK1(K46N)
(Plasmid #22897)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22897 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pME18s
  • Backbone size w/o insert (bp) 3410
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    a novel protein kinase structurally related to C. elegans UNC-51
  • Alt name
    ULK1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3614
  • Mutation
    changed Lysine 46 to Aspargine
  • GenBank ID
    AF053756
  • Entrez Gene
    Ulk1 (a.k.a. AU041434, Unc51.1, mKIAA0722)
  • Tag / Fusion Protein
    • 3xHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME18S-3HA-mULK1(K46N) was a gift from Masaaki Muramatsu (Addgene plasmid # 22897 ; http://n2t.net/addgene:22897 ; RRID:Addgene_22897)
  • For your References section:

    Identification of mouse ULK1, a novel protein kinase structurally related to C. elegans UNC-51. Yan J, Kuroyanagi H, Kuroiwa A, Matsuda Y, Tokumitsu H, Tomoda T, Shirasawa T, Muramatsu M. Biochem Biophys Res Commun. 1998 May 8. 246(1):222-7. 10.1006/bbrc.1998.8546 PubMed 9600096