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pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC
(Plasmid #22931)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22931 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Modifications to backbone
    Created by removing last 3aa from pcDNA3-HA-PHLPP1 N-terminal truncation (Addgene Plasmid #22404)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PHLPP1-deltaC
  • Alt name
    SCOP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3606
  • Mutation
    PHLPP-deltaC, deletion of PDZ binding domain--the last three amino acid residues 1203-1205.
  • Entrez Gene
    PHLPP1 (a.k.a. PHLPP, PLEKHE1, PPM3A, SCOP)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid was created using PHLPP1 N-terminal truncation as template. Please note that PHLPP1 N-terminal truncation was referred to as PHLPP1alpha in the original publication (Gao et al. Mol Cell 2015)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC was a gift from Alexandra Newton (Addgene plasmid # 22931 ; http://n2t.net/addgene:22931 ; RRID:Addgene_22931)
  • For your References section:

    PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth. Gao T, Furnari F, Newton AC. Mol Cell. 2005 Apr 1. 18(1):13-24. 10.1016/j.molcel.2005.03.008 PubMed 15808505