Addgene - pCF CREB M1 Plasmid Data

Plasmid 22969: pCF CREB M1

Gene/insert name:
CREB
Species:
R. norvegicus (rat)
Entrez Gene:
Creb1 (Creb)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Mutation:
Changed Serine 133 to Alanine
Vector backbone:
pCF
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5000
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Marc Montminy
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Characterization of a CREB gain-of-function mutant with constitutive transcriptional activity in vivo. Du et al (Mol Cell Biol. 2000 Jun . 20(12):4320-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22969" in your Materials and Methods section.