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Addgene

pEGFPC1-6XHis-FLKSRP
(Plasmid #23001)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 23001 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KSRP
  • Species
    H. sapiens (human)
  • Mutation
    valine inserted at aa#2
  • Entrez Gene
    KHSRP (a.k.a. FBP2, FUBP2, KSRP, p75)
  • Tags / Fusion Proteins
    • EGFP (N terminal on insert)
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The KHSRP insert in this plasmid contains an extra amino acid, valine, at position #2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPC1-6XHis-FLKSRP was a gift from Douglas Black (Addgene plasmid # 23001 ; http://n2t.net/addgene:23001 ; RRID:Addgene_23001)
  • For your References section:

    Differentiation-induced colocalization of the KH-type splicing regulatory protein with polypyrimidine tract binding protein and the c-src pre-mRNA. Hall MP, Huang S, Black DL. Mol Biol Cell. 2004 Feb . 15(2):774-86. 10.1091/mbc.e03-09-0692 PubMed 14657238