Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pDUP 4-5
(Plasmid #23023)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 23023 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    DUP33
  • Backbone manufacturer
    Z Dominski and R Kole
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    c-src
  • Alt name
    src
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Src (a.k.a. pp60c-src)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NsiI (not destroyed)
  • 3′ cloning site DraIII (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DUP4-5 (Fig. 2A) was made by digesting a src minigene construct (Fig. 1A)
with ApaI and BamHI and inserting the fragment containing the N1 exon into
DUP4-1 (Addgene Plasmid 23022) at the ApaI and BglII sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDUP 4-5 was a gift from Douglas Black (Addgene plasmid # 23023 ; http://n2t.net/addgene:23023 ; RRID:Addgene_23023)
  • For your References section:

    A complex intronic splicing enhancer from the c-src pre-mRNA activates inclusion of a heterologous exon. Modafferi EF, Black DL. Mol Cell Biol. 1997 Nov . 17(11):6537-45. PubMed 9343417