pBud-EGFP
(Plasmid #23027)

• Depositing Lab: Andrea LeBlanc
• Publication: Jodoin et al PLoS One. 2009 . 4(8):e6647.

Backbone

• Vector backbone: pBudCE4.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4578
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP
• Insert Size (bp): 718
• Entrez Gene: eGFP (a.k.a. pPRS3a_01)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: As described in Roucou X, Giannopoulos PN, Zhang Y, Jodoin J, Goodyer CG, LeBlanc AC. Cellular prion protein inhibits proapoptotic Bax conformational change in human neurons and in breast carcinoma MCF-7 cells. Cell Death Differ. 2005, 12(7):783-795.
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Bigenic vector with EF-1alpha promoter and CMV promoter