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pDEST26-hOPTN-D474N
(Plasmid #23051)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 23051 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST26
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Optineurin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1733
  • Mutation
    D474N
  • Entrez Gene
    OPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR (unknown if destroyed)
  • 3′ cloning site attR (unknown if destroyed)
  • 5′ sequencing primer CMVPro Fwd
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST26-hOPTN-D474N was a gift from Jon Ashwell (Addgene plasmid # 23051 ; http://n2t.net/addgene:23051 ; RRID:Addgene_23051)
  • For your References section:

    Optineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP. Zhu G, Wu CJ, Zhao Y, Ashwell JD. Curr Biol. 2007 Aug 21. 17(16):1438-43. 10.1016/j.cub.2007.07.041 PubMed 17702576