Addgene - pCMV-Tag2B Flag BimEL(TD) Plasmid Data

Plasmid 23092: pCMV-Tag2B Flag BimEL(TD)

Gene/insert name:
Bim EL (TD)
Alt name:
Bim
Insert size:
590
Species:
M. musculus (mouse)
Entrez Gene:
Bcl2l11 (RP23-134I15.1, 1500006F24Rik, Bim, Bod)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Mutation:
Changed Threonine 112 to Aspartate
Vector backbone:
pCMV-Tag2B
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4300
Cloning site 5':
Bam HI
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
cmv F List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Roger Davis
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Multisite phosphorylation regulates Bim stability and apoptotic activity. Hubner et al (Mol Cell. 2008 May 23. 30(4):415-25. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 23092" in your Materials and Methods section.