Gene/insert name: Tipin
Alt name: Timeless-interacting protein
Insert size: 903
Species:
H. sapiens (human)
Entrez Gene:
TIPIN
(FLJ20516)
Fusion protein or tag: Flag
Terminal: N terminal on insert
Mutation: Changed Leucine 195 to Alanine
Vector backbone:
pcDNA5/FRT/TOSearch Vector Database )
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5137
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
CMV: CGCAAATGGGCGGTAGGCGTG
List of Sequencing Primers
3' sequencing primer:
BGH
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence:
View sequences (2)
Principal Investigator:
Aziz Sancar
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
23118" in your Materials and Methods section.
