|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||23118||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5137
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameTimeless-interacting protein
SpeciesH. sapiens (human)
Insert Size (bp)903
MutationChanged Leucine 195 to Alanine
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV: CGCAAATGGGCGGTAGGCGTG
- 3′ sequencing primer BGH (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA5/FRT/TO-Flag-Tipin-L195A was a gift from Aziz Sancar (Addgene plasmid # 23118)
For your References section:Tipin-RPA interaction mediates Chk1 phosphorylation by ATR in response to genotoxic stress. Kemp MG, Akan Z, Yilmaz S, Grillo M, Smith-Roe SL, Kang TH, Cordeiro-Stone M, Kaufmann WK, Abraham RT, Sancar A, Unsal-Kacmaz K. J Biol Chem. 2010 Mar 15. ():. 10.1074/jbc.M110.110304 PubMed 20233725
Generated by Addgene from plasmid data.