Addgene - pFastBac-His-Tipin-L195A Plasmid Data

Plasmid 23121: pFastBac-His-Tipin-L195A

Gene/insert name:
Tipin
Alt name:
Timeless-interacting protein
Insert size:
903
Species:
H. sapiens (human)
Entrez Gene:
TIPIN (FLJ20516)
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Mutation:
Changed Leucine 195 to Alanine
Vector backbone:
pFastBacHTb
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Insect Expression
Backbone size w/o insert (bp):
4856
Cloning site 5':
XbaI
Site destroyed during cloning:
Unknown
Cloning site 3':
KpnI
Site destroyed during cloning:
Unknown
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Gentamicin
Sequence: View sequences (2)
Principal Investigator:
Aziz Sancar
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Tipin-RPA interaction mediates Chk1 phosphorylation by ATR in response to genotoxic stress. Kemp et al (J Biol Chem. 2010 Mar 15. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 23121" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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