pHR'CMVGFP_hRIF1(1701-2446)IREShygro
(Plasmid #23137)

• Depositing Lab: Lifeng Xu
• Publication: Xu et al J Cell Biol. 2004 Dec 6. 167(5):819-30.

Backbone

• Vector backbone: pHR'CMVGFPIREShygro_WSIN18
• Backbone manufacturer: Didier Trono Lab
• Backbone size w/o insert (bp): 10000
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: Grow in DH5a @ 30C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RIF1 deletion construct
• Alt name: GFP_hRIF1(1701-2446)
• Species: H. sapiens (human)
• Insert Size (bp): 3093
• Entrez Gene: RIF1
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CAGAGCTCGTTTAGTGAACCGTCAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Backbone vector sequence is only approximated. Please contact the Trono lab for full vector sequence.