p817-mgammaFcry-mCherry
(Plasmid #23154)

• Depositing Lab: Zbynek Kozmik
• Publication: Vopalensky et al Biotechniques. 2010 Mar . 48(3):235-6.

Backbone

• Vector backbone: p817 (pBSII derivate)
• Vector type: multicloning site flanked by I-SceI sites

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Growth instructions: DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mouse gammaF crystallin promoter:mCherry
• Alt name: Crygf
• Species: M. musculus (mouse)
• Insert Size (bp): 284
• Mutation: mouse gammaF crystallin promoter (-225 - +73) preceeding mCherry-SV40pA
• Entrez Gene: Crygf (a.k.a. Len-2, Cryg-2, DGcry-2, 3110001K11Rik)
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: I-SceI (not destroyed)
• 3′ cloning site: I-SceI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mCherry was cloned from pCS2. The original p817 vector was obtained from Jochen Wittbrodt (University of Heidelberg)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A=>G and G=>A mismatches in gene do not have any functional significance.