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p5954 pcDNA-Xpress-His-USP15 C269A
(Plasmid #23217)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 23217 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA-4C
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    USP15
  • Alt name
    ubiquitin carboxyl-terminal hydrolase 15 isoform 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    702
  • Mutation
    C269A
  • GenBank ID
    NP_006304.1
  • Entrez Gene
    USP15 (a.k.a. UNPH-2, UNPH4)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • Xpress (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5954 pcDNA-Xpress-His-USP15 C269A was a gift from Peter Howley (Addgene plasmid # 23217 ; http://n2t.net/addgene:23217 ; RRID:Addgene_23217)
  • For your References section:

    The ubiquitin-specific peptidase USP15 regulates human papillomavirus type 16 E6 protein stability. Vos RM, Altreuter J, White EA, Howley PM. J Virol. 2009 Sep . 83(17):8885-92. 10.1128/JVI.00605-09 PubMed 19553310