Addgene - Sox17 Flox Plasmid Data

Plasmid 23335: Sox17 Flox

Gene/insert name:
Sex determining region Y-box containing gene 17
Alt name:
Sox17
Insert size:
4299
Species:
M. musculus (mouse)
GenBank ID:
NM_011441
Entrez Gene:
Sox17 (Sox)
Mutation:
None
Vector backbone:
ploxPFLPneo
(Search Vector Database)
Vector type:
Mouse Targeting
Backbone size w/o insert (bp):
3000
Cloning site 5':
Xho1
Site destroyed during cloning:
No
Cloning site 3':
Hinc2
Site destroyed during cloning:
Yes
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Sean Morrison
Terms and Licenses:
MTA

Comments:

The source of ploxPFLPneo is
Hiraoka et al Mol Cell Biol 26, 6139.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Sox17 dependence distinguishes the transcriptional regulation of fetal from adult hematopoietic stem cells. Kim et al (Cell. 2007 Aug 10. 130(3):470-83. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 23335" in your Materials and Methods section.