Addgene - pDONR223-FGFR2 Plasmid Data

Plasmid 23904: pDONR223-FGFR2

Gene/insert name:
FGFR2
Alt name:
fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte growth factor receptor, craniofacial dysostosis 1,
Insert size:
2469
Species:
H. sapiens (human)
Entrez Gene:
FGFR2 (BBDS, BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25)
Mutation:
A52D
Vector backbone:
pDONR223
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Gateway Donor vector
Backbone size w/o insert (bp):
5005
Cloning method:
Gateway Cloning
5' sequencing primer:
M13pUC-fwd List of Sequencing Primers
3' sequencing primer:
M13rev
Bacterial resistance(s)
Spectinomycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
William Hahn
Principal Investigator:
David Root
Terms and Licenses:
MTA

Comments:

The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.

A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Johannessen et al (Nature. 2010 Nov 24. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 23904" in your Materials and Methods section.