Addgene - pJEL061 Plasmid Data

Plasmid 24016: pJEL061

Gene/insert name:
None
Fusion protein or tag:
EYFP
Terminal:
C terminal on backbone
Vector backbone:
pLEW82
(Search Vector Database)
Backbone manufacturer:
George Cross
Vector type:
Trypanosome Expression
Backbone size (bp):
5000
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers, other:
Phleomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
George Cross
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

Target locus: rRNA spacer

Regulation: 1 tet Op 2 T7 terminators

For additional information see George Cross's website at http://tryps.rockefeller.edu/trypsru2_plasmids....

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24016" in your Materials and Methods section.