Addgene - pyrFEKO-PUR Plasmid Data

Plasmid 24021: pyrFEKO-PUR

Gene/insert name:
None
Vector backbone:
pyrFEKO
(Search Vector Database)
Vector type:
Floxed targeting vector for Trypanosome
Backbone size (bp):
5753
Cloning site 5':
BamHI/PvuII
Site destroyed during cloning:
No
Cloning site 3':
SbfI/HindIII
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
George Cross
Terms and Licenses:
MTA

Comments:

Unpublished (variant of constructs described in Scahill et al 2008).

For more information about using this vector, please see: http://tryps.rockefeller.edu/trypsru2_cre-lox.html

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24021" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

24024: pyrFEKO-BSD

24019: pLEW100cre_del_tetO

24020: pyrFEKO-HYG