|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24086||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
Insert Size (bp)7100
Entrez GeneKidins220 (a.k.a. ARMS)
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site SpeI (destroyed during cloning)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
pFLAG-ARMS was generated by ligating two fragments of ARMS- HindIII/KpnI and KpnI/SpeI-- into the HindIII and XbaI sites of pFLAG. The HindIII site was generated by PCR;note that the SpeI and Xba I sites no longer exist after the ligation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFLAG ARMS was a gift from Moses Chao (Addgene plasmid # 24086 ; http://n2t.net/addgene:24086 ; RRID:Addgene_24086)
For your References section:An evolutionarily conserved transmembrane protein that is a novel downstream target of neurotrophin and ephrin receptors. Kong H, Boulter J, Weber JL, Lai C, Chao MV. J Neurosci. 2001 Jan 1. 21(1):176-85. PubMed 11150334