Addgene - GFP-DRD3 Plasmid Data

Plasmid 24098: GFP-DRD3

Gene/insert name:
DRD3
Species:
H. sapiens (human)
Entrez Gene:
Drd3 ()
Fusion protein or tag:
alpha7 nicotinic signal peptide
Terminal:
N terminal on backbone
Fusion protein or tag:
eGFP
Terminal:
N terminal on backbone
Vector backbone:
pCEP4
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
10200
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
SV40pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Jean-Michel Arrang
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Interactions of GIPC with dopamine D2, D3 but not D4 receptors define a novel mode of regulation of G protein-coupled receptors. Jeanneteau et al (Mol Biol Cell. 2004 Feb . 15(2):696-705. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24098" in your Materials and Methods section.