Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

GFP-DRD3
(Plasmid #24098)

Add to Cart
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCEP4
  • Backbone size w/o insert (bp) 10200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    DRD3
  • Species
    H. sapiens (human)
  • Entrez Gene
    Drd3
  • Tags / Fusion Proteins
    • alpha7 nicotinic signal peptide (N terminal on backbone)
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-DRD3 was a gift from Jean-Michel Arrang (Addgene plasmid # 24098)
  • For your References section:

    Interactions of GIPC with dopamine D2, D3 but not D4 receptors define a novel mode of regulation of G protein-coupled receptors. Jeanneteau F, Diaz J, Sokoloff P, Griffon N. Mol Biol Cell. 2004 Feb . 15(2):696-705. 10.1091/mbc.E03-05-0293 PubMed 14617818