Addgene - PCMV4-3 HA/IkB-alpha(SS32,36AA) Plasmid Data

Plasmid 24143: PCMV4-3 HA/IkB-alpha(SS32,36AA)

Gene/insert name:
IkB alpha
Species:
H. sapiens (human)
Entrez Gene:
NFKBIA (IKBA, MAD-3, NFKBI)
Fusion protein or tag:
3xHA
Terminal:
N terminal on insert
Mutation:
S32A S36A
Vector backbone:
pCMV4-3xHA
(Search Vector Database)
Backbone manufacturer:
Green lab
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4874
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
hGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Warner Greene
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Both amino- and carboxyl-terminal sequences within I kappa B alpha regulate its inducible degradation. Sun et al (Mol Cell Biol. 1996 Mar . 16(3):1058-65. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24143" in your Materials and Methods section.