peZinCh-NB
(Plasmid
#24173)
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24173 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepECFP-C1
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3900
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsNova Blue (for DNA amplification)
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameeZinCh-NB
-
Alt nameCerulean-linker-Citrine
-
Alt nameCer-L9-Cit
-
Insert Size (bp)1800
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer CMV terminator (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
peZinCh-NB was a gift from Maarten Merkx (Addgene plasmid # 24173 ; http://n2t.net/addgene:24173 ; RRID:Addgene_24173) -
For your References section:
Genetically encoded FRET sensors to monitor intracellular Zn(2+) homeostasis. Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M. Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368. 10.1038/nmeth.1368 PubMed 19718032
Map uploaded by the depositor.
