pCMV E2F1 E132
(Plasmid #24224)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCMVNeoBam
  • Backbone size w/o insert (bp) 6200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    E2F1 E132
  • Alt name
    E2F-1
  • Alt name
    E2F1
  • Species
    H. sapiens (human)
  • Entrez Gene
    E2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site - (unknown if destroyed)
  • 3′ cloning site - (unknown if destroyed)
  • 5′ sequencing primer Bglob-intron-F
  • 3′ sequencing primer Bglob-pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Recloned from D. Cress CMV Invitrogen

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV E2F1 E132 was a gift from Kristian Helin (Addgene plasmid # 24224)
  • For your References section:

    Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation. Helin K, Wu CL, Fattaey AR, Lees JA, Dynlacht BD, Ngwu C, Harlow E. Genes Dev. 1993 Oct . 7(10):1850-61. 10.1101/gad.7.10.1850 PubMed 8405995