Addgene - pCMV E2F1 E132 Plasmid Data

Plasmid 24224: pCMV E2F1 E132

Gene/insert name:
E2F1 E132
Alt name:
E2F-1
Alt name:
E2F1
Species:
H. sapiens (human)
Entrez Gene:
E2F1 (E2F-1, RBAP1, RBBP3, RBP3)
Vector backbone:
pCMVNeoBam
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6200
Cloning site 5':
-
Site destroyed during cloning:
Unknown
Cloning site 3':
-
Site destroyed during cloning:
Unknown
5' sequencing primer:
Bglob-intron-F List of Sequencing Primers
3' sequencing primer:
Bglob-pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Kristian Helin
Terms and Licenses:
MTA

Comments:

Recloned from D. Cress CMV Invitrogen

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation. Helin et al (Genes Dev. 1993 Oct . 7(10):1850-61. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24224" in your Materials and Methods section.