Addgene - pCMVHA EED wt Plasmid Data

Plasmid 24231: pCMVHA EED wt

Gene/insert name:
EED
Species:
H. sapiens (human)
Entrez Gene:
EED (HEED, WAIT1)
SUZ12 (CHET9, JJAZ1)
EZH2 (ENX-1, ENX1, EZH1, KMT6, KMT6A, WVS, WVS2)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pCMVHA
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6600
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
BamHI
Site destroyed during cloning:
Unknown
5' sequencing primer:
Bglob-intron-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Kristian Helin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: EZH2 is downstream of the pRB-E2F pathway, essential for proliferation and amplified in cancer. Bracken et al (EMBO J. 2003 Oct 15. 22(20):5323-35. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24231" in your Materials and Methods section.