Addgene - pCI-neo-Myc-hAtg14 Plasmid Data

Plasmid 24293: pCI-neo-Myc-hAtg14

Gene/insert name:
autophagy related genes 14
Alt name:
Atg14
Insert size:
1400
Species:
H. sapiens (human)
GenBank ID:
AK131251
Fusion protein or tag:
3xMyc
Terminal:
N terminal on backbone
Vector backbone:
pCI-neo Myc
(Search Vector Database)
Backbone manufacturer:
Promega(original) + Myc
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5472
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
FLJ16178,obtained from the Department of Biotechnology, National Institute of Technology and Evaluation, Tokyo, Japan
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Noboru Mizushima
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Itakura et al (Mol Biol Cell. 2008 Dec . 19(12):5360-72. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24293" in your Materials and Methods section.