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pCI-neo-Myc-hAtg14
(Plasmid #24293)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCI-neo Myc
  • Backbone manufacturer
    Promega(original) + Myc
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    autophagy related genes 14
  • Alt name
    Atg14
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • GenBank ID
    AK131251
  • Tag / Fusion Protein
    • 3xMyc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    FLJ16178,obtained from the Department of Biotechnology, National Institute of Technology and Evaluation, Tokyo, Japan
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo-Myc-hAtg14 was a gift from Noboru Mizushima (Addgene plasmid # 24293)
  • For your References section:

    Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Itakura E, Kishi C, Inoue K, Mizushima N. Mol Biol Cell. 2008 Dec . 19(12):5360-72. 10.1091/mbc.E08-01-0080 PubMed 18843052