Addgene - p3xFLAG-CMV14-mULK1 Plasmid Data

Plasmid 24301: p3xFLAG-CMV14-mULK1

Gene/insert name:
a novel protein kinase structurally related to C. elegans UNC-51
Alt name:
ULK1
Insert size:
3614
Species:
M. musculus (mouse)
GenBank ID:
AF053756
Entrez Gene:
Ulk1 (AU041434, Unc51.1, mKIAA0722)
Fusion protein or tag:
3xFLAG
Terminal:
C terminal on backbone
Vector backbone:
p3xFLAG-CMV14
(Search Vector Database)
Backbone manufacturer:
sigma-aldrich
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6299
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
CMV 30 sequencing primer List of Sequencing Primers
3' sequencing primer:
CMV 24 sequencing primer
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Noboru Mizushima
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Hosokawa et al (Mol Biol Cell. 2009 Apr . 20(7):1981-91. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24301" in your Materials and Methods section.