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EdTP
(Plasmid #24311)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pRRLSIN.cPPT.PGK-GFP.WPRE
  • Backbone manufacturer
    Naldini/Trono (Addgene #12252)
  • Backbone size w/o insert (bp) 6074
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    EF1alpha-dnhTcf4 // SV40-PuroR
  • Alt name
    Tcf4
  • Alt name
    dominant negative tcf4
  • Alt name
    puromycin N-acetyltransferase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4114
  • Mutation
    31 first amino acids of hTcf4 have been deleted.
  • GenBank ID
    NM_030756
  • Entrez Gene
    TCF7L2 (a.k.a. TCF-4, TCF4)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (vec) and AvaII (insert) (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GGCCCGAAGGAATAGAAG
  • 3′ sequencing primer AACGGGCCACAACTCCTC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The dominant-negative, flag tagged Tcf4 was obtained from Amanda Mikels)
  • Terms and Licenses

Depositor Comments

This plasmid requires a 2nd generation lentiviral packaging system.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EdTP was a gift from Roel Nusse (Addgene plasmid # 24311)
  • For your References section:

    Lentiviral vectors to probe and manipulate the wnt signaling pathway. Fuerer C, Nusse R. PLoS One. 2010 . 5(2):e9370. 10.1371/journal.pone.0009370 PubMed 20186325