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pGEX-GST-AGO3
(Plasmid #24318)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24318 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AGO3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Entrez Gene
    AGO3 (a.k.a. EIF2C3)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-GST-AGO3 was a gift from Carl Novina (Addgene plasmid # 24318)
  • For your References section:

    Distinct passenger strand and mRNA cleavage activities of human Argonaute proteins. Wang B, Li S, Qi HH, Chowdhury D, Shi Y, Novina CD. Nat Struct Mol Biol. 2009 Dec . 16(12):1259-66. 10.1038/nsmb.1712 PubMed 19946268