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pmCD8-GFP,y+
(Plasmid #24350)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24350 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Yellow gene
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    9000
  • Tag / Fusion Protein
    • CD8-GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer GFP-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This enhancer trap vector is based on the pGalW vector (Gerlitz et al., 2002). The
GAL4 insert from pGalW was removed by complete NotI and partial XbaI digestion and replaced
with the GAL80 ORF isolated as a NotI/XbaI fragment from pCasper-tubP-GAL80 (Lee and Luo,
1999), to generate the plasmid pG80,w+. To generate pG80,y+, the white gene from pG80,w+ was
excised by EcoRV/SacII digestion, the vector was blunted with Klenow, and replaced with the
yellow gene as a blunted SalI fragment from the y S/G plasmid (a gift from Pamela Geyer,
University of Iowa). To generate pmCD8-GFP,y+, the GAL80 insert was excised by NotI digestion
and replaced with mCD8-GFP which was PCR amplified from pUAST-mCD8GFP to include 5' and
3' NotI restriction sites (see also Berdnik et al., 2006).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCD8-GFP,y+ was a gift from Liqun Luo (Addgene plasmid # 24350 ; http://n2t.net/addgene:24350 ; RRID:Addgene_24350)
  • For your References section:

    The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990