Addgene - pQUAST-shibire (ts1) Plasmid Data

Plasmid 24362: pQUAST-shibire (ts1)

Gene/insert name:
Shibire (ts1)
Insert size:
2500
Species:
D. melanogaster (fly)
Entrez Gene:
shi (Dmel_CG18102, CG18102, DNM3, DYN, Ddyn, Ddyn3, Ddyn4, Dmel\CG18102, Dyn, Shi, anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, dDyn, dyn, l(1)VII, l(1)shi, shibere)
Vector backbone:
pQUAST
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
9000
Cloning site 5':
NotI
Site destroyed during cloning:
Unknown
Cloning site 3':
KpnI
Site destroyed during cloning:
Unknown
5' sequencing primer:
pCasper-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Map:
View map
Supplemental document:
pQUAST-shibire (ts1) (GenBank)
Principal Investigator:
Liqun Luo
Terms and Licenses:
MTA

Comments:

Shibire was PCR amplified from genomic DNA of UAS-shibire transgenic flies (Kitamoto, 2001) using genUASFOR and genUASREVsv40 oligos, and ligated into the NotI/KpnI sites of pQUAST.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis Potter et al (Volume 141, Issue 3, 536-548, 30 April 2010 PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24362" in your Materials and Methods section.