FLAG-FOXO3 6A
(Plasmid #24382)

• Depositing Lab: Anne Brunet
• Publication: Greer et al J Biol Chem. 2007 Oct 12. 282(41):30107-19.

Backbone

• Vector backbone: pECE
• Backbone size w/o insert (bp): 2900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FOXO3
• Species: H. sapiens (human)
• Insert Size (bp): 2038
• Mutation: T179A, S399A, S413A, S555A, S588A, and S626A
• Entrez Gene: FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: SV40pro-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The wild-type version of FOXO3 is also available at Addgene, plasmid 8360.