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(Plasmid #24429)


Item Catalog # Description Quantity Price (USD)
Plasmid 24429 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
    mutations in extracellular loops 2 (see comments below) and 3 (E297Q), making it a RASSL.
  • Entrez Gene
    OPRK1 (a.k.a. K-OR-1, KOP, KOR, KOR-1, KOR1, OPRK)
  • Tags / Fusion Proteins
    • GFP (N terminal on insert)
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Extracellular Loop 2 (EL2) mutations shown in lowercase: aSSVGISAIVLGvTqpRdgavvCtLQFPspswyWDtvt

For more information about this construct see:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Rog was a gift from Bruce Conklin (Addgene plasmid # 24429 ; ; RRID:Addgene_24429)
  • For your References section:

    Engineered G protein coupled receptors reveal independent regulation of internalization, desensitization and acute signaling. Scearce-Levie K, Lieberman MD, Elliott HH, Conklin BR. BMC Biol. 2005 . 3():3. 10.1186/1741-7007-3-3 PubMed 15707483