|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24429||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Mutationmutations in extracellular loops 2 (see comments below) and 3 (E297Q), making it a RASSL.
Entrez GeneOPRK1 (a.k.a. K-OR-1, KOP, KOR, KOR-1, KOR1, OPRK)
/ Fusion Proteins
- GFP (N terminal on insert)
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F, EGFP-C
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Extracellular Loop 2 (EL2) mutations shown in lowercase: aSSVGISAIVLGvTqpRdgavvCtLQFPspswyWDtvt
For more information about this construct see: http://openwetware.org/wiki/R03:GFP-tagged_RASSL
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-Rog was a gift from Bruce Conklin (Addgene plasmid # 24429 ; http://n2t.net/addgene:24429 ; RRID:Addgene_24429)
For your References section:Engineered G protein coupled receptors reveal independent regulation of internalization, desensitization and acute signaling. Scearce-Levie K, Lieberman MD, Elliott HH, Conklin BR. BMC Biol. 2005 . 3():3. 10.1186/1741-7007-3-3 PubMed 15707483