Addgene - pcDNA3.1hSIRT4

Plasmid 24482: pcDNA3.1hSIRT4_HA

Gene/insert name:
Sirtuin 4
Alt name:
SIRT4
Insert size:
1000
Species:
H. sapiens (human)
GenBank ID:
NM_012240
Entrez Gene:
SIRT4 (SIR2L4, MGC57437, MGC130046, MGC130047)
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Vector backbone:
pcDNA3.1
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Eric Verdin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation. Hirschey et al (Nature. 2010 Mar 4. 464(7285):121-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24482" in your Materials and Methods section.