Addgene - pcDNA3.1hLCAD_FLAG Plasmid Data

Plasmid 24491: pcDNA3.1hLCAD_FLAG

Gene/insert name:
Long chain acyl-CoA dehydrogenase
Alt name:
LCAD
Alt name:
acadl
Insert size:
1300
Species:
H. sapiens (human)
GenBank ID:
BC039063
Entrez Gene:
ACADL (LCAD, ACAD4, FLJ94052)
Fusion protein or tag:
FLAG
Terminal:
C terminal on insert
Vector backbone:
pcDNA3.1
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5700
Cloning site 5':
KnnI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Eric Verdin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation. Hirschey et al (Nature. 2010 Mar 4. 464(7285):121-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24491" in your Materials and Methods section.