|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24510||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7434
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Kanamycin
Growth Strain(s)ccdB Survival
Growth instructionsccdB resistance E. Coli like Invitrogen’s One Shot® ccdB Survival™-T1R Chemically Competent Cells
Copy numberHigh Copy
Gene/Insert nameYn, Yc, ECFP, EYFP
Alt nameCFP, YFP, ECFP, EYFP
SpeciesAequorea victoria (Jellyfish)
Insert Size (bp)716
/ Fusion Proteins
- ECFP (C terminal on backbone)
- EYFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site clonase (destroyed during cloning)
- 3′ cloning site recombinase (destroyed during cloning)
- 5′ sequencing primer E LoxP-Rcmb GGCCTCGTACTACGCCTATT
- 3′ sequencing primer TCCGGATGAGCATTCATCAG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made by1. He, L. et al. Flow cytometric measurement of fluorescence (Forster) resonance energy transfer from cyan fluorescent protein to yellow fluorescent protein using single-laser excitation at 458 nm. Cytometry A. 53, 39-54 (2003).
Terms and Licenses
Note: This plasmid has been partially sequenced. Due to the presence of repeated features in the plasmid that prohibit thorough sequencing, the provided sequence should be considered theoretical and could contain errors.
Please refer to Lu, Jian-Ping, Beatty, Laura, and Pinthus, Jehonathan . Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2008.1550.2 > (2008)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Y pFe-YcLoxP-YnAtt was a gift from Jehonathan Pinthus (Addgene plasmid # 24510 ; http://n2t.net/addgene:24510 ; RRID:Addgene_24510)