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Y pFe-YcLoxP-YnAtt
(Plasmid #24510)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24510 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pVitro2
  • Backbone manufacturer
    Invivogen
  • Backbone size w/o insert (bp) 7434
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB resistance E. Coli like Invitrogen’s One Shot® ccdB Survival™-T1R Chemically Competent Cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Yn, Yc, ECFP, EYFP
  • Alt name
    CFP, YFP, ECFP, EYFP
  • Species
    Aequorea victoria (Jellyfish)
  • Insert Size (bp)
    716
  • GenBank ID
    P42212
  • Tags / Fusion Proteins
    • ECFP (C terminal on backbone)
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site clonase (destroyed during cloning)
  • 3′ cloning site recombinase (destroyed during cloning)
  • 5′ sequencing primer E LoxP-Rcmb GGCCTCGTACTACGCCTATT
  • 3′ sequencing primer TCCGGATGAGCATTCATCAG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    1. He, L. et al. Flow cytometric measurement of fluorescence (Forster) resonance energy transfer from cyan fluorescent protein to yellow fluorescent protein using single-laser excitation at 458 nm. Cytometry A. 53, 39-54 (2003).
  • Terms and Licenses

Depositor Comments

Note: This plasmid has been partially sequenced. Due to the presence of repeated features in the plasmid that prohibit thorough sequencing, the provided sequence should be considered theoretical and could contain errors.

Please refer to Lu, Jian-Ping, Beatty, Laura, and Pinthus, Jehonathan . Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2008.1550.2 > (2008)

http://precedings.nature.com/documents/1550/version/2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Y pFe-YcLoxP-YnAtt was a gift from Jehonathan Pinthus (Addgene plasmid # 24510 ; http://n2t.net/addgene:24510 ; RRID:Addgene_24510)