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pECFP-DEVG-Venus
(Plasmid #24538)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24538 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pECFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ECFP-DEVG-Venus
  • Alt name
    Uncleavable caspase FRET reporter control
  • Species
    Synthetic
  • Insert Size (bp)
    792
  • Mutation
    DEVDR mutated to DEVG
  • Promoter CMV
  • Tags / Fusion Proteins
    • ECFP
    • Venus

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspEI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

DEVG sequence in linker prevents cleavage by caspases as control for pECFP-DEVDR-Venus (Addgene plasmid #24537).

constructed by ligating Venus (YFP) between BamHI and EcoRI sites in pECFP-C1 and ligating linkers encoding cleavage sequences as BspEI-BamHI fragments between ECFP and Venus. Multiple serine and glycine residues flanking the cleavage sequences were added to increase linker flexibility and substrate accessibility. See https://www.ncbi.nlm.nih.gov/pubmed/18406323 for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pECFP-DEVG-Venus was a gift from Peter Sorger (Addgene plasmid # 24538 ; http://n2t.net/addgene:24538 ; RRID:Addgene_24538)
  • For your References section:

    Modeling a snap-action, variable-delay switch controlling extrinsic cell death. Albeck JG, Burke JM, Spencer SL, Lauffenburger DA, Sorger PK. PLoS Biol. 2008 Dec 2. 6(12):2831-52. 10.1371/journal.pbio.0060299 PubMed 19053173