Addgene - pRRL CAG NFlag BAF170 ires GFP Plasmid Data

Plasmid 24562: pRRL CAG NFlag BAF170 ires GFP

Gene/insert name:
BAF155
Alt name:
smarcc2
Insert size:
3600
Species:
M. musculus (mouse)
GenBank ID:
NM_001114097
Entrez Gene:
Smarcc2 (5930405J04Rik)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pRRL-sin18PPT CAG-ires-GFP
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
9200
Cloning site 5':
Age1
Site destroyed during cloning:
No
Cloning site 3':
Pst1
Site destroyed during cloning:
No
5' sequencing primer:
gctaaccatgttcatgccttc List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL10 Gold
Growth temperature (℃):
37
Growth instructions:
XL-10 Gold, Stratagene
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
Jerry Crabtree
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An embryonic stem cell chromatin remodeling complex, esBAF, is essential for embryonic stem cell self-renewal and pluripotency. Ho et al (Proc Natl Acad Sci U S A. 2009 Mar 31. 106(13):5181-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24562" in your Materials and Methods section.