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pGADT7-ADH700-yeCherry-pTEF1(ashbya)-yeGFP-DHFR
(Plasmid #24585)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24585 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pADH1 yeCherry::pTEF1 (Ashbya)yeGFP-DHFR
  • Alt name
    ADH1 promoter ye Cherry
  • Alt name
    pTEF1 (Ashbya) yeGFP-ecDHFR
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3000
  • Mutation
    1. Truncated version of ADH700 promoter (SbfI/SpeI) driven yeast enhanced RFP (SpeI/-). 2. promoter TEF1(Ashbya.) (SacII/BglII) driven yeast enhanced GFP fused to E.coli DHFR (BglII/XhoI)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site sbf1 (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGADT7-ADH700-yeCherry-pTEF1(ashbya)-yeGFP-DHFR was a gift from Thomas Wandless (Addgene plasmid # 24585)
  • For your References section:

    Dicistronic regulation of fluorescent proteins in the budding yeast Saccharomyces cerevisiae. Edwards SR, Wandless TJ. Yeast. 2009 Dec 17. ():. 10.1002/yea.1744 PubMed 20017217