|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24588||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsDB3.1 or ccdB resistance strain. We do not allow E. coli harbouring this plasmid to grow to saturation (no longer than 14 hours). We prefer to grow it at 30C and 180RPM of shaking when applicable.
Copy numberLow Copy
Gene/Insert nameGateway(TM) cassette
Insert Size (bp)1705
/ Fusion Protein
- VA (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (destroyed during cloning)
- 3′ cloning site attR2 (destroyed during cloning)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
The CMV promoter from the pLJM1 vector (Addgene) was PCR amplified and cloned into pLD-puro-EcVA using NdeI/MluI to generate pLD-puro-CcVA.
pLD-puro-EcVA (pLD-puromycin resistance- EF1alpha, C-terminal VA tag) was generated as described in the paper from pLKO.1
VA tag= versatile affinity tag, 3XFlag+6XHis+StrepIII
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLD-puro-CcVA was a gift from Jason Moffat (Addgene plasmid # 24588)
For your References section:A lentiviral-based functional proteomics approach identifies chromatin remodelling complexes important for the induction of pluripotency. Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Punna T, Isserlin R, Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J. Mol Cell Proteomics. 2010 Mar 19. ():. 10.1074/mcp.M000002-MCP201 PubMed 20305087